Colorimetric method for estimating methylmalonic acid in urine.
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چکیده
منابع مشابه
Colorimetric method for estimating methylmalonic acid in urine.
The increased urinary excretion of methylmalonic acid in vitamin B12 deficiency has attracted attention and several studies have suggested that it has clinical value as a test of vitamin B12 deficiency (Cox and White, 1962; Bashir, Hinterberger, and Jones, 1966). Chromatographic methods of estimation have been used but are either time consuming or only semiquantitative over a narrow range of me...
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FTIPPURIC ACID 1S an important metabolic product normally present in urine in amounts determined by tile ingestion of benzyl-containing foods and medicinals (ii). Because this compound is comparatively inert chemically, identification requires isolation and the determination of physical constants after extensive purification. There has long been need for a reliable and sensitive color reaction ...
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We describe a gas-chromatographic procedure for determining methylmalonic acid in serum and urine. The sample is acidified and the compound is extracted from it with diethyl ether, derivatized with N,Obis(trimethylsilyl)trifluoroacetamide, and gas chromatographed. Precision and sensitivity of the method were verified by recovery studies and by comparing the resultswith those foran established p...
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Total urinary protein is rapidly precipitated at room temperature by tannic acid. The tannic acid/protein precipitate, dissolved in aqueous triethanolamine/ferric chloride solution, gives a purple-violet color of high absorptivity. Absorbance at 510 nm is linearly related to concentration from 0.05 to 1.50 A for a protein content of 0.05 to 1.50 g/liter, and less than 5 mg/liter can be detected...
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In the course of feeding experiments in which mono-, di-, and trimethylamines were fed to dogs, it was desirable to determine the urinary excretion of methylamine (monomethylamine). The available methods were found to be cumbersome and not well adapted to the routine analysis of a large number of urines. Weber and Wilson (1) separated ammonia from the amines by the use of yellow mercuric oxide ...
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ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1968
ISSN: 0021-9746
DOI: 10.1136/jcp.21.2.221